Introduction

In the following sections, it is explained how to work with GRIDLib and some example analyses are shown. However, if you decide to perform GRID analysis, it is highly recommended to read the paper where the original authors present the ideas and the mathematics behind GRID:

Reisser, M., Hettich, J., Kuhn, T., Popp, A.P., Große-Berkenbusch, A. and Gebhardt, J.C.M. (2020). Inferring quantity and qualities of superimposed reaction rates from single molecule survival time distributions. Scientific Reports, 10(1). doi:10.1038/s41598-020-58634-y.

Glossary

During the rest of the guide, some terms of the original paper are used. Here is a glossary of some of those terms:

Survival time distribution

A survival time distribution is a function that gives the probability that an object of interest will survive past a certain time.

GRID

Abbreviation for Genuine Rate IDentification.

Integration time

The time that the laser and camera are turned on during an acquisition. (\(\tau_{\mathrm{int}}\))

Dark time

The time that the laser and camera are turned off between acquisitions. (\(\tau_{\mathrm{d}}\))

Time-lapse time

The sum of the integration time and the dark time. (\(\tau_{\mathrm{tl}}\))

Photobleaching rate

The rate at which photobleaching happens. (\(k_{\mathrm{b}}\))

Photobleaching number

The photobleaching rate multiplied by the integration time. (\(a=k_{\mathrm{b}}\times\tau_{\mathrm{int}}\))